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Khulna (Bengali: খুলনা ) is the third-largest city in Bangladesh after Dhaka and Chittagong. It is located in the southern west area of Bangladesh and situated on the banks of the Rupsha and Bhairab Rivers. It is the headquarters of Khulna District and Khulna Division. The river port of Khulna is one of the oldest river ports in Bangladesh.

Often referred to as Industrial City (Bengali: শিল্প নগরী ), it is one of the important industrial and commercial areas of the country. It has a sea port named Mongla on its outskirts, 48 km south from Khulna city, which is the second seaport (after Chittagong seaport) in Bangladesh. Khulna is also the gateway to the Sundarbans, the largest tidal forest in the world.

The population under the jurisdiction of the City Corporation was 1,000,000 in 2007 estimation. The wider metropolitan area had at the same time an estimated population of 1,435,422.

The city is 333 km (by road) southwest from the natural capital of Bangladesh, Dhaka, a 133 km beeline. The cities are connected by road, air, railway and waterways. The steamboat fleet for this route includes the ancient ships Tern (1912), Osrich and Lepcha.